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CHIR99021 was removed after 72 h and DE cells were cultured in RPMI/B27 + Insulin for 24 h ( For growth factor Definitive Endoderm (DE) differentiation, human iPSCs cultured in 6-well dishes to 60% confluence were washed sequentially with DPBS / and DMEM/FI2 medium (Thermo Fisher Scientific)

Downregulation of the hepatocystin encoded by PRKCSH results in dysfunctional glucosidase II, and thereby increase in autophagy via mTOR dependent pathway 74

Murine models were inoculated with murine B16 melanoma (B16M) to obtain in vivo metastatic melanoma that was subsequently tested with the above-stated combination treatments

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